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╬ťOUNT project - Heraklion Bay Sampling

Latest version published by Hellenic Center for Marine Research on Nov 19, 2021 Hellenic Center for Marine Research

The present study was conducted in the continental shelf of Heraklion Bay in depths from 50 to 200 meters, covering an area exposed to wave action. The coastal zone is affected by small-scale freshwater inputs and urban waste discharges while the offshore system is characterised by marked oligotrophy. The study area is located outside the local bottom trawling fishing grounds.

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How to cite

Researchers should cite this work as follows:

Panayota (Yolanda) Koulouri, Heraklion Bay Sampling, MOUNT Project, 01-06 June 2019.

Rights

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The publisher and rights holder of this work is Hellenic Center for Marine Research. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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Keywords

Metadata; benthic boundary layer macrofauna; hyperbenthos; macrobenthos; macrozooplankton; benthic crustaceans; continental shelf; Heraklion Bay; eastern Mediterranean

Contacts

Who created the resource:

Yolanda Koulouri
Principal Researcher
Hellenic Centre for Marine Research Former U.S. Base of Gournes 71500 Heraklion Crete GR +30 2810 337716

Who can answer questions about the resource:

Yolanda Koulouri
Principal Researcher
Hellenic Centre for Marine Research Former U.S. Base of Gournes 71500 Heraklion Crete GR +30 2810 337716

Who filled in the metadata:

Dimitra Mavraki
Data Manager
Hellenic Centre for Marine Research Former U.S. Base of Gournes 71500 Heraklion Crete GR +302810337740

Who else was associated with the resource:

Content Provider
Costas Dounas
Research Director
Hellenic Centre for Marine Research Former U.S. Base of Gournes 71500 Heraklion Crete GR +30 2810337814
Originator
Apostolos Krystallas
Technician
Hellenic Centre for Marine Research Former U.S. Base of Gournes 71500 Heraklion Crete GR
Originator
Maria Maidanou
Technician
Hellenic Centre for Marine Research Former U.S. Base of Gournes 71500 Heraklion Crete GR +302810337842

Geographic Coverage

Sampling was carried out in one transect of three stations (St.1, St. 2, St. 3) at depths of 50,100 and 200 m. St. 1 Lat. 35.362000, Lon. 25.1000667 / St. 2 Lat. 35. 382883, Lon. 25.099350 / St. 3 Lat. 35. 422783, Lon. 25.094817

Bounding Coordinates South West [35.264, 24.78], North East [35.528, 25.659]

Taxonomic Coverage

hyperbenthic, (endo-)benthic and planktonic macrofaunal taxa (species of crustaceans, polychaets, molluscs)

Phylum  Mollusca
Subphylum  Crustacea
Class  Polychaeta

Temporal Coverage

Start Date / End Date 2019-06-01 / 2019-06-04

Project Data

Development and application of innovative methodologies for the study of Benthic Boundary Layer. The benthic boundary layer (BBL) supports epibenthic, hyperbenthic and zooplanktonic, mostly macrofaunal, organisms with different degrees of mobility and bottom dependence. BBL species constitute a very important link in marine food webs as consumers of detritus particles and associate microbes, meiobenthos and plankton and as prey for demersal fish and epibenthic crustaceans, many of which are commercially important. Nevertheless, the significance of this particular macrofauna is underestimated, especially in studies of benthic-pelagic coupling related to energy fluxes and also in holistic approaches of the marine ecosystem, mainly due to the difficulties in accessibility of this habitat. A research team of IMBBC has developed new sampling gears for the study of BBL habitat focused mainly on the sampling of macrofauna as well as the simultaneous measurement of relevant physical and biogeochemical parametres. These methodologies will contribute towards a better understanding of the role of BBL biodiversity with respect to nutrient regeneration, carbon cycling and energy transfer to higher trophic levels.

Title MOdern UNifying Trends in marine biology
Identifier PA (Partnership Agreement for the Development Framework) 2014-2020: 5002470
Funding EPAnEK 2014-2020, Operational Programme, Competitiveness, Entrepreneurship, Innovation.
Study Area Description The present study was conducted in the continental shelf of Heraklion Bay in depths from 50 to 200 metres.
Design Description One field survey was conducted on the continental shelf of Heraklion Bay. Sampling was carried out in one transect of three stations (St.1, St. 2, St. 3) at depths of 50,100 and 200 m. At each station: -the physical properties of the water column (temperature, salinity, photosynthetically active radiation (PAR)) were measured by means of a Sea-Bird Electronics (SBE-19) CTD system - water samples were collected by using Niskin bottles (5L) within 0.5 m above the seabed and a relatively new bottom water sampling method (Dounas, 2006) for the determination of chlorophyll a (Chl a), phaeopigments (Phaeo), particulate organic carbon (POC) concentrations. Samples were filtered through Whatman GF/F glass fibre filters on board, frozen immediately and stored at 20oC and analysed upon return to the laboratory. - one surface sediment sample (0-1 cm) was taken by using Smith McIntyre grab (0.10m2, sampling surface) for grain size analysis performed according to Buchanan (1984) and for the determination of Chl-a, phaeopigments and POC concentrations and stored at 20oC for subsequent laboratory analysis. Chloroplastic pigments concentrations in both sediment and water column samples were determined according to the fluorometric method of Yentsch and Menzel (1963), Lorenzen and Jeffrey (1980), using a Turner 112 fluorometer. POC measurements were determined according to the method of Hedges and Stern (1984), using a Perkin Elmer CHN 2400 analyzer. -three tows were carried out by the hyperbenthic sledge modified in the framework of MOUNT project. Hyperbenthic samples were collected during daylight hours. -five sediment samples were taken using a Smith McIntyre grab (0.10 m2). -five plankton samples were taken by means of a WP2 plankton net (0.5 mm mesh size) from a depth ranging from ~3 m above the seabed throughout the water column to the water surface. Biological samples were preserved in 90% alcohol on board immediately after collection and sorted under a dissecting microscope upon return to the laboratory.

The personnel involved in the project:

Point Of Contact
Yolanda Koulouri

Sampling Methods

1.the physical properties of the water column were measured by means of a Sea-Bird Electronics (SBE-25) CTD system 2.water samples were collected by using Niskin bottles (5L) and by using a new bottom water sampling method (Dounas, 2006) 3.surface sediment samples (0-1 cm) were taken by using Smith McIntyre grab (0.10m2, sampling surface) 4. hyperbenthic samples were carried out by the hyperbenthic sledge modified in the framework of MOUNT project 5.endobenthic samples were taken by using a Smith McIntyre grab (0.10 m2) 6.macroplanktonic samples were taken by means of a WP2 plankton net (0.5 mm mesh size)

Study Extent One field survey (1-4 June 2019) was conducted on the continental shelf of Heraklion Bay. Sampling was carried out in one transect of three stations (St.1, St. 2, St. 3) at depths of 50,100 and 200 m.
Quality Control Taxonomy mapped according to WoRMS and locations according to Marine Regions

Method step description:

  1. Sampling was carried out in one transect of three stations at depths of 50,100 and 200 m. At each station: -the physical properties of the water column (temperature, salinity, photosynthetically active radiation (PAR)) were measured by means of a Sea-Bird Electronics (SBE-19) CTD system - water samples were collected by using Niskin bottles (5L) within 0.5 m above the seabed and a relatively new bottom water sampling method (Dounas, 2006) for the determination of chlorophyll a (Chl a), phaeopigments (Phaeo), particulate organic carbon (POC) concentrations. Samples were filtered through Whatman GF/F glass fibre filters on board, frozen immediately and stored at 20oC and analysed upon return to the laboratory. - one surface sediment sample (0-1 cm) was taken by using Smith McIntyre grab (0.10m2, sampling surface) for grain size analysis performed according to Buchanan (1984) and for the determination of Chl-a, phaeopigments and POC concentrations and stored at 20oC for subsequent laboratory analysis. Chloroplastic pigments concentrations in both sediment and water column samples were determined according to the fluorometric method of Yentsch and Menzel (1963), Lorenzen and Jeffrey (1980), using a Turner 112 fluorometer. POC measurements were determined according to the method of Hedges and Stern (1984), using a Perkin Elmer CHN 2400 analyzer. -three tows were carried out by the hyperbenthic sledge modified in the framework of MOUNT project. Hyperbenthic samples were collected during daylight hours. -five sediment samples were taken using a Smith McIntyre grab (0.10 m2). -five plankton samples were taken by means of a WP2 plankton net (0.5 mm mesh size) from a depth ranging from ~3 m above the seabed throughout the water column to the water surface. Biological samples were preserved in 90% alcohol on board immediately after collection and sorted under a dissecting microscope upon return to the laboratory.

Additional Metadata

Alternative Identifiers http://ipt.medobis.eu/resource?r=mount